Rabbit Anti-phospho-SHC1 (Ser36) antibody |
反应物种(预测) |
Dog,Pig,Cow,Horse |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500, |
研究领域 |
肿瘤,细胞生物,免疫学,信号转导,转录调节因子,线抗体, |
标签 |
Array |
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Sample:
Lane1: Brain(Rat) Lysate at 30 ug
Lane2: Heart(Rat) Lysate at 30 ug
Primary: Anti-phospho-SHC(Ser36) (bs-3410R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 63kD
Observed band size : 63kD
RRID:AB_10884010
产品名称:Rabbit Anti-phospho-SHC1 (Ser36) antibody
别名: SHC (phospho S36); p-SHC (phospho S36); p-SHC(Ser36); p46; p52; p52 SHC; p52SHC; p66; p66 SHC; p66SHC; SH2 domain protein C1; SHC (Src homology 2 domain containing) transforming protein 1; SHC 1; SHC A; Shc; SHC transforming protein 1; SHC transforming pr
中文名称:磷酸化SH2结构域转化蛋白1抗体
英文名称:Rabbit Anti-phospho-SHC1 (Ser36) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated Synthesised phosphopeptide derived from human SHC around the phosphorylation site of Ser36
抗原表位:LP(p-S)PS
SWISS:P29353
Gene ID :6464
Human Gene ID:6464
This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
Function:Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosin
Subunit:Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR in vitro via the PID domain. Once activated, binds to GRB2. Interacts with tyrosine-phosphorylated CD3T and DDR2. Interacts with the N-terminal region of APS. Interacts with phosphory
Subcellular Location:Cytoplasm.
Isoform p46Shc: Mitochondrion matrix. Note=Localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52S
Tissue Specificity:Widely expressed. Expressed in neural stem cells but absent in mature neurons.
Post-translational modifications:Phosphorylated by activated epidermal growth factor receptor. Phosphorylated in response to FLT4 and KIT signaling. Isoform p46Shc and isoform p52Shc are phosphorylated on tyrosine residues of the Pro-rich domain. Isoform p66Shc is phosphorylated on Ser-3
Similarity:Contains 1 PID domain.
Contains 1 SH2 domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.