Rabbit Anti-SAHH antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-SAHH antibody

多克隆 | SKU：bs-5029R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-5029R

￥1098.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-SAHH antibody
反应物种（预测）	Human,Chicken,Dog,Pig,Cow,Horse,Rabbit
产品应用（已验证）	WB
产品应用（可尝试）	IHC,IF,ELISA,IP
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IP=1:20-100,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,
研究领域	肿瘤,细胞生物,免疫学,信号转导,转录调节因子
标签	Array

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SAHH Polyclonal Antibody, Unconjugated(bs-5029R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
SAHH was immunoprecipitated from mouse kidney tissue with bs-5029R at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution.
Lane 1: mouse kidney tissue lysate 10 µg (Input).
Lane 2: bs-5029R IP in mouse kidney tissue lysate.
Lane 3: native rabbit IgG IP in mouse kidney tissue lysate (negative control).
Secondary
All lanes : Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000
Sample:
Kidney (Mouse) Lysate at 40 ug
Skin (Mouse) Lysate at 40 ug
Primary: Anti- SAHH (bs-5029R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD

产品信息

RRID：AB_10856783
产品名称：Rabbit Anti-SAHH antibody
别名： Adenosylhomocysteinase; AdoHcyase; ahcY; S adenosyl L homocysteine hydrolase; S adenosylhomocysteine hydrolase; S-adenosyl-L-homocysteine hydrolase; SAHH; SAHH_HUMAN.
中文名称：S腺苷L-同型半胱氨酸水解酶抗体
英文名称：Rabbit Anti-SAHH antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human SAHH
抗原表位：25-125/432
SWISS：P23526
Gene ID ：191
Human Gene ID：191

产品介绍

Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
Function：Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of ade
Subunit：Homotetramer.
Subcellular Location：Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
DISEASE：Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD) [MIM:613752]. A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial d
Similarity：Belongs to the adenosylhomocysteinase family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.