Rabbit Anti-SRXN1 antibody |
反应物种(预测) |
Chicken,Dog,Pig,Cow,Horse,Rabbit |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200, |
研究领域 |
细胞生物,免疫学 |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (SRXN1) Polyclonal/MonoclonalAntibody, Unconjugated (bs-8329R) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Rat) Lysate at 40 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Lane 4: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-SRXN1 (bs-8329R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 15 kD
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Sample: Kidney (Mouse) Lysate at 40 ug
Primary: Anti-SRXN1 (bs-8329R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 15 kD
Observed band size: 15 kD
RRID:RRID
产品名称:Rabbit Anti-SRXN1 antibody
别名: C20orf1392; Chromosome 20 open reading frame 139; dJ850E9.2; Npn3; Npn31; SRX1; SRXN 1; Sulfiredoxin 1; Sulfiredoxin 1 homolog (S. cerevisiae); Sulfiredoxin 1 homolog; YKL086W; SRXN1_HUMAN.
中文名称:抗氧化蛋白1抗体
英文名称:Rabbit Anti-SRXN1 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human SRXN1
抗原表位:45-137/137
SWISS:Q9BYN0
Gene ID :140809
Human Gene ID:140809
Sulfiredoxin, also designated Sulfiredoxin-1 and chromosome 20 open reading frame 139 (C20orf139), is a cytoplasmic antioxidant protein involved in signaling through catalytic reduction of oxidative modifications. It regulates peroxiredoxins (PRXs), a family of proteins that reduce hydroperoxides, by reducing the conserved cysteine from sulfinic to sulfenic acid. This impacts the role of PRX in the reduction of other downstream transcription factors and kinase signaling pathways. The Sulfiredoxin protein specifically acts on the PRDX1, PRDX2, PRDX3 and PRDX4 peroxiredoxins, but not on PRDX5 or PRDX6. Sulfiredoxin acts as a phosphotransferase and an athioltransferase and is widely expressed, with highest levels detected in lung, spleen, kidney and thymus tissues.
Function:SRXN1 contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in the peroxiredoxins PRDX1, PRDX2, PRDX3 and PRDX4. It does not act on PRDX5 or PRDX6. SRXN1 may catalyze the reduction in a multi-step
Subcellular Location:Cytoplasmic
Tissue Specificity:Widely expressed with highest levels in kidney, lung, spleen and thymus.
Similarity:Belongs to the sulfiredoxin family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.