Rabbit Anti-S100A13 antibody |
反应物种(预测) |
Rat,Dog,Cow,Horse,Rabbit |
产品应用(已验证) |
IHC,FCM |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=2ug/Test,IF=1:100-500, |
研究领域 |
肿瘤 |
标签 |
Array |
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Blank control:A431.
Primary Antibody (green line): Rabbit Anti-S100A13 antibody (bs-2617R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-S100A13 Polyclonal Antibody, Unconjugated(bs-2617R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
RRID:AB_10857869
产品名称:Rabbit Anti-S100A13 antibody
别名: Protein S100 A13; S100 calcium binding protein A13; S10AD_HUMAN; Protein S100-A13; S100 calcium-binding protein A13; S100a13.
中文名称:S100A13抗体
英文名称:Rabbit Anti-S100A13 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆,分泌型蛋白
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human S100A13
抗原表位:51-98/98
SWISS:Q99584
Gene ID :6284
Human Gene ID:6284
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is widely expressed in various types of tissues with a high expression level in thyroid gland. In smooth muscle cells, this protein co-expresses with other family members in the nucleus and in stress fibers, suggesting diverse functions in signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Function:Plays a role in the export of proteins that lack a signal peptide and are secreted by an alternative pathway. Binds two calcium ions per subunit. Binds one copper ion. Binding of one copper ion does not interfere with calcium binding. Required for the cop
Subunit:Homodimer. Part of a copper-dependent multiprotein complex containing S100A13, FGF1 and SYT1. Interacts with FGF1 and SYT1.
Subcellular Location:Cytoplasm. Secreted. Secretion is mediated by exposure to stress and requires copper ions.
Tissue Specificity:Expressed in heart and skeletal muscle.
Similarity:Belongs to the S-100 family.
Contains 1 EF-hand domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.