| Rabbit Anti-Hsc70 antibody |
| 反应物种（预测） |
Chicken,Pig,Rabbit |
| 产品应用（已验证） |
IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/test,IF=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,免疫学,信号转导,转录调节因子 |
| 标签 |
Array |
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Blank control:U-2OS.
Primary Antibody (green line): Rabbit Anti-Hsc70 antibody (bs-5117R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Hsc70) Polyclonal Antibody, Unconjugated (bs-5117R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Hsc70) Polyclonal Antibody, Unconjugated (bs-5117R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
RRID：AB_10855389
产品名称：Rabbit Anti-Hsc70 antibody
别名： 2410008N15Rik;
Constitutive heat shock protein 70;
Heat shock 70 kDa protein 8;
Heat shock 70kD protein 10;
Heat shock 70kD protein 8;
Heat shock 70kDa protein 8;
Heat shock cognate 71 kDa protein;
Heat shock cognate protein 54;
Heat shock cognate
中文名称：热休克蛋白71抗体
英文名称：Rabbit Anti-Hsc70 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human HSP71
抗原表位：501-600/646
SWISS：P11142
Gene ID ：3312
Human Gene ID：3312
This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Function：Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
Subunit：Interacts with HSPH1/HSP105. Interacts with IRAK1BP1 (By similarity). Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1,
Subcellular Location：Cytoplasm. Melanosome.
Tissue Specificity：Ubiquitous.
Post-translational modifications：Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated.
Similarity：Belongs to the heat shock protein 70 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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