Rabbit Anti-BRN3A antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-BRN3A antibody

多克隆 | SKU：bs-3669R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-3669R

￥1098.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-BRN3A antibody
反应物种（预测）	Chicken,Dog,Pig,Cow,Rabbit
产品应用（已验证）	WB,IHC,FCM
产品应用（可尝试）	IF,ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/test,IF=1:100-500,
研究领域	免疫学,神经生物学,转录调节因子
标签	Array

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BRN3A Polyclonal Antibody, Unconjugated(bs-0071R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Jurkat.
Primary Antibody (green line): Rabbit Anti-BRN3A antibody (bs-3669R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Spinal cord (Mouse) Lysate at 40 ug
Primary: Anti-BRN3A (bs-3669R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 33 kD
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BRN3A Antibody(bs-3669R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

产品信息

RRID：AB_10857817
产品名称：Rabbit Anti-BRN3A antibody
别名： Brain specific homeobox/POU domain protein 3A; Brn 3.0; BRN 3A; Brn3; Homeobox/POU domain protein RDC 1; Homeobox/POU domain protein RDC1; Oct T1; OctT1; POU Domain Class 4 Transcription Factor 1; POU4F1; RDC 1; RDC1.
中文名称：转录因子Brn3蛋白抗体
英文名称：Rabbit Anti-BRN3A antibody
中文别名：Brn3α;
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human BRN3A
抗原表位：325-419/419
SWISS：Q01851
Gene ID ：5457
Human Gene ID：5457

产品介绍

This gene encodes a member of the POU-IV class of neural transcription factors. This protein is expressed in a subset of retinal ganglion cells and may be involved in the developing sensory nervous system. This protein may also promote the growth of cervical tumors. A translocation of this gene is associated with some adult acute myeloid leukemias. [provided by RefSeq, Mar 2012].
Function：Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
Subcellular Location：Nucleus.
Tissue Specificity：Brain. Seems to be specific to the retina. Present in the developing brain, spinal cord and eye.
DISEASE：Expression peaks early in embryogenesis (day 13.5) and is undetectable 14 days after birth.
Similarity：Belongs to the POU transcription factor family. Class-4 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.