| Rabbit Anti-IL-4R antibody |
| 反应物种（预测） |
Mouse,Rat,Pig,Horse |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=1μg /test, |
| 研究领域 |
细胞生物,免疫学,细胞膜受体,细胞表面分子, |
| 标签 |
Array |
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Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug
Lane 2: 293T (Human) Cell Lysate at 30 ug
Lane 3: Raji (Human) Cell Lysate at 30 ug
Lane 4: Jurkat (Human) Cell Lysate at 30 ug
Primary: Anti-IL-4R (bs-22901R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140-160 kD
Observed band size: 150 kD
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Sample:
Lane 1: IL-4R-His protein (1-232) Overexpression 293T (Human) Cell Lysate at 4 ug
Lane 2: IL-4R-His protein (1-232) Overexpression 293T (Human) Cell Lysate at 4 ug
Primary:
Lane 1: Anti-IL-4R (bs-22901R) at 1/1000 dilution
Lane 2: Anti-His tag (bsm-33004M) at 1/1000 dilution
Secondary:
Lane 1: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Lane 2: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 43-48 kD
Observed band size: 43-48 kD
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Blank control: Raji (blue).
Primary Antibody:Rabbit Anti-IL-4R antibody(bs-2458R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (bs-2458R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
RRID：AB_10853366
产品名称：Rabbit Anti-IL-4R antibody
别名： CD124;
IL 4R alpha;
IL-4 receptor subunit alpha;
IL-4-binding protein;
IL-4R subunit alpha;
IL-4R-alpha;
IL-4RA;
IL4-BP;
Il4r;
IL4R nirs variant 1;
IL4RA;
IL4RA_HUMAN;
Interleukin 4 receptor alpha chain;
Interleukin 4 receptor;
sIL4Ralpha/pr
中文名称：白细胞介素4受体抗体
英文名称：Rabbit Anti-IL-4R antibody
中文别名：IL-4Rα; IL 4Rα; IL-4 Rα; IL-4-Rα;
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human IL-4R
抗原表位：201-300/825
抗原细胞定位：Extracellular
SWISS：P24394
Gene ID ：3566
Human Gene ID：3566
This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Two transcript variants encoding different isoforms, a membrane-bound and a soluble form, have been found for this gene. [provided by RefSeq, Jul 2008].
Function：Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production
Subunit：The functional IL4 receptor is formed by initial binding of IL4 to IL4R. Subsequent recruitment to the complex of the common gamma chain, in immune cells, creates a type I receptor and, in non-immune cells, of IL13RA1 forms a type II receptor. IL4R can al
Subcellular Location：Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.
Tissue Specificity：Isoform 1 and isoform 2 are highly expressed in activated T-cells.
Post-translational modifications：On IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction.
The soluble form (sIL4R/IL4BP) can also be produced by proteolyt
Similarity：Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 1 fibronectin type-III domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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