Rabbit Anti-Tbx21 antibody |
反应物种(预测) |
Rat,Dog,Pig,Cow,Horse |
产品应用(已验证) |
WB |
产品应用(可尝试) |
IHC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
肿瘤,细胞生物,免疫学,信号转导,细胞凋亡,转录调节因子 |
标签 |
Array |
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Sample:
Lane 1: Lung (Mouse) Lysate at 40 ug
Lane 2: Thymus (Mouse) Lysate at 40 ug
Primary: Anti-Tbx21 (bs-3599R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 60 kD
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Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Tbx21/T-bet Polyclonal Antibody, Unconjugated(bs-3599R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti-Tbx21 (bs-3599R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD
RRID:AB_10883956
产品名称:Rabbit Anti-Tbx21 antibody
别名: T bet; T box 21; T box expressed in T cells; T box protein 21; T box transcription factor TBX21; T cell specific T box transcription factor; T cell specific T box transcription factor T bet; T PET; TBET; TBLYM; TBX 21; TBX21; TPET; Transcription factor TB
中文名称:T细胞介导的转录调节因子Tbx21抗体
英文名称:Rabbit Anti-Tbx21 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human Tbx21
抗原表位:201-300/535
SWISS:Q9UL17
Gene ID :30009
Human Gene ID:30009
This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq, Jul 2008].
Function:Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs.
Subcellular Location:Nucleus.
Tissue Specificity:T-cell specific.
DISEASE:Genetic variations in TBX21 are associated with susceptibility to asthma with nasal polyps and aspirin intolerance (ANPAI) [MIM:208550]. A condition consisting of asthma, aspirin sensitivity and nasal polyposis. Nasal polyposis is due to chronic inflammat
Similarity:Contains 1 T-box DNA-binding domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.