| Rabbit Anti-TLR7 antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Horse |
| 产品应用（已验证） |
WB |
| 产品应用（可尝试） |
IHC,ICC,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500, |
| 研究领域 |
细胞生物,免疫学,表观遗传学, |
| 标签 |
Array |
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Sample:
Brain (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-TLR7 (bs-6601R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 121 kD
Observed band size: 121 kD
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Tissue/cell: human placenta tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TLR7 Polyclonal Antibody, Unconjugated(bs-6601R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
RRID：AB_11090775
产品名称：Rabbit Anti-TLR7 antibody
别名： TLR-7; PRO285; TLR 7; TLR7_HUMAN; Toll like receptor 7; Toll-like receptor 7; UNQ248.
中文名称：Toll样受体7抗体
英文名称：Rabbit Anti-TLR7 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human TLR7
抗原表位：461-560/1049
抗原细胞定位：Extracellular
SWISS：Q9NYK1
Gene ID ：51284
Human Gene ID：51284
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Function：Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-st
Subunit：Interacts with MYD88 via their respective TIR domains. Interacts with UNC93B1.
Subcellular Location：Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endosome. Lysosome. Cytoplasmic vesicle, phagosome. Note=Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
Tissue Specificity：Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.
Similarity：Belongs to the Toll-like receptor family.
Contains 27 LRR (leucine-rich) repeats.
Contains 1 TIR domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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