Rabbit Anti-ABCB11 antibody |
反应物种(预测) |
Human,Rabbit |
产品应用(已验证) |
IHC |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500, |
研究领域 |
肿瘤,细胞生物,免疫学,转录调节因子,通道蛋白 |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABCB11 ) Polyclonal Antibody, Unconjugated (bs-1954R) at 1:300 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABCB11 ) Polyclonal Antibody, Unconjugated (bs-1954R) at 1:300 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID:AB_10855138
产品名称:Rabbit Anti-ABCB11 antibody
别名: ABC member 16, MDR/TAP subfamily; ABCBB_HUMAN; ATP binding cassette sub family B (MDR/TAP) member 11; ATP binding cassette sub family B member 11; ATP-binding cassette sub-family B member 11; Bile salt export pump; BRIC2; Bsep; PGY4; progressive familial
中文名称:胆汁酸盐输出泵/ATP结合盒转运蛋白11抗体
英文名称:Rabbit Anti-ABCB11 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human BSEP
抗原表位:21-120/1321
SWISS:Q9QY30
Gene ID :8647
Human Gene ID:8647
ATP-binding cassette (ABC) transporters are an evolutionarily conserved family of proteins that catalyze the transport of molecules across extra- and intracellular membranes through the energy of ATP hydrolysis. ABC genes comprise seven subfamilies, designated ABC1, Mdr/TAP, MRP, ALD, OABP, GCN20 and White. The secretion of bile salt molecules from blood into bile is a major driving force for bile formation. Bile salt export pump (BSEP) is a member of the Mdr/TAP subfamily of ABC transporters that mediates the transport of bile acids across the hepatocyte canalicular membrane and regulates bile acid-dependent bile secretion. BSEP contains putative phosphorylation sites for protein kinase A, protein kinase C (PKC) and Ca2+-calmodulin dependent kinase II, whose regulation may be dependent on bile salt concentration.
Function:Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes.
Subunit:Interacts with HAX1.
Subcellular Location:Membrane; Multi-pass membrane protein.
Tissue Specificity:Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ.
DISEASE:Defects in ABCB11 are the cause of progressive familial intrahepatic cholestasis type 2 (PFIC2) [MIM:601847]. PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defec
Similarity:Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.