货号 |
bs-8502R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-caspase-9 p10 antibody |
中文名称 |
Rabbit Anti-caspase-9 p10 antibody |
研究领域 |
细胞生物,免疫学,神经生物学,细胞凋亡 |
英文别名 |
APAF 3; APAF3; Apoptosis related cysteine peptidase; Apoptotic protease activating factor 3; Apoptotic protease MCH 6; Apoptotic protease MCH6 antibody
CASP 9; CASP9; Caspase 9; Caspase 9 apoptosis related cysteine protease; Caspase 9 precursor; Caspase 9 |
反应物种(已验证) |
Human,Mouse,Rat |
反应物种(预测) |
Chicken,Dog,Pig,Cow,Horse,Rabbit |
产品应用(已验证) |
WB,IHC,FCM |
产品应用(可推荐) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg /Test,IF=1:50-200, |
克隆类型 |
多克隆 |
抗体来源 |
Rabbit |
理论分子量 |
10/50 |
细胞定位 |
分泌型蛋白 |
性状 |
Liquid |
免疫原 |
KLH conjugated synthetic peptide derived from human caspase-9 subunit p10 |
抗原表位 |
351-416/416 |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein A |
Tissue |
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. |
SIMILARITY |
Belongs to the peptidase C14A family.
Contains 1 CARD domain. |
SUBUNIT |
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cyt |
Function |
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).< |
Post-translational |
Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to blo |
SWISS |
P55211 |
Gene ID |
842 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is comprised of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6, caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9 and caspase-10. Ced-3/caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly(ADP-ribose) polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. Caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli. |