货号 |
bs-0924R-1 |
品牌 |
|
浓度 |
|
货期 |
现货 |
英文名称 |
Rabbit Anti-Mafa antibody |
中文名称 |
Rabbit Anti-Mafa antibody |
研究领域 |
神经生物学,信号转导,转录调节因子,糖尿病,内分泌病, |
英文别名 |
Mafa homolog; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A;
Pancreatic beta-cell-specific transcriptional activator; (avian)(V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; MAFA_MOUSE. |
反应物种(已验证) |
Rat |
反应物种(预测) |
Human,Mouse |
产品应用(已验证) |
WB,IHC |
产品应用(可推荐) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
克隆类型 |
多克隆 |
抗体来源 |
Rabbit |
理论分子量 |
37 |
细胞定位 |
细胞核 |
性状 |
Liquid |
免疫原 |
KLH conjugated synthetic peptide derived from mouse Mafa |
抗原表位 |
265-359/359 |
亚型 |
IgG |
纯化方法 |
affinity purified by Protein A |
SUBCELLULAR |
Nucleus. Note=Detected in nuclei of pancreas islet beta cells. |
Tissue |
Selectively expressed in pancreatic beta but not in alpha cells (at protein level). Expressed in eyes and at low levels in thymus. Expressed in brain, lung, spleen and kidney. Expressed in embryo. |
SIMILARITY |
Belongs to the bZIP family. Maf subfamily. Contains 1 bZIP (basic-leucine zipper) domain. |
SUBUNIT |
Binds DNA as a homodimer. Interacts with PCAF. Interacts with NEUROD1 and PDX1. |
Function |
Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Regulate |
Post-translational |
Ubiquitinated, leading to its degradation by the proteasome.
Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation incr |
SWISS |
Q8CF90 |
Gene ID |
378435 |
保存条件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Important Note |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
英文介绍 |
Insulin gene expression is regulated by several islet-enriched transcription factors. However, MAFA is the only beta cell-specific activator. MAFA selectively induces endogenous insulin transcription in non-beta cells. MAFA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MAFA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development. |